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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene continues to be observed in many tumors, which leads to chromosome instability, and so may well lead to tumorigenesis. This protein was identified to associate with, and become associated with, the phosphorylation of NPAT protein (nuclear protein mapped towards the ATM locus), which participates in mobile-cycle regulated histone gene expression and plays a significant job in advertising and marketing cell-cycle development from the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, are already described. Two more splice variants had been described but thorough nucleotide sequence information and facts will not be however out there.
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Blocking peptides are peptides that bind specifically to your focus on antibody and block antibody binding. These peptide normally contains the epitope acknowledged because of the antibody.
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This manual outlines further possible optimization of mobile and immunoassay parameters to be sure the best possible results are...
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This guidebook outlines more attainable optimization of mobile and immunoassay parameters to be sure the absolute best effects are...
Antibodies sure to the blocking peptide no more bind to your epitope to the focus on website protein. By evaluating the staining through the blocked antibody compared to the antibody on your own, one can see which staining is specific.
Concentrate on Information and facts Cyclin E belongs into the remarkably conserved cyclin relatives, whose customers are characterized by a dramatic periodicity in protein abundance in the mobile cycle. Cyclins operate as regulators of CDK kinases. Distinct cyclins exhibit distinctive expression and degradation designs which contribute into the temporal coordination of every mitotic celebration. Cyclin E sorts a fancy with and features as being a regulatory subunit of CDK2, whose exercise is needed for cell cycle G1/S changeover. This protein accumulates on the G1-S phase boundary and is particularly degraded as cells progress through S period.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by each antibodies...
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